| Core Facilities Adult Stem Cell Core
Location | Collection Sites | Services | Charges | Forms | Contact
Purpose and Overview
To propagate, expand and supply high quality samples of normal mesenchymal stem cells (MSCs) from in vitro cultures with appropriate quality control assurances for use by LCRC investigators who are studying any aspect of MSCs in relationship to cancer.
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Location
3rd floor of the Tulane School of Medicine Building room 3163 in the Department of Structural and Cellular Biology.
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Collection Sites
MSCs are purchased from the GMP facility of Tulane Center for Gene Therapy as frozen vials at early passage (P1).
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Services
MSC Propagation and Expansion
MSC Propagation in vitro: 106 MSCs will be cultured until a total of 107 cells are obtained (10-14 days). Cells will then be distributed to LCRC investigators (See Cost Below).
Colony Forming Unit (CFU) Assay
The CFU assay assures MSC quality by testing the ability of MSCs to generate single-cell derived colonies, also known as colony forming units. Good quality, early passage hMSC’s expanded at low density should yield a CFU assay of greater than 40%. If requested, the CFU assay will be carried out at the time of harvest and results will be available after 14 days.
(See Cost Below)
Differentiation Assays
The differentiation assays assure MSC quality by testing MSCs ability to retain multi-potentiality for differentiation along different lineages. We test for adipogenesis and osteogenesis of p2 MSC’s. If requested, the differentiation assays will be carried out at the time of harvest and the results will be available 23-30 days later.
(See Cost Below)
Stem Cell Storage
MSC’s may be stored in the liquid nitrogen storage system for investigators that would like to store specific donor cells.
(Cost not yet determined)
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Charges
| Discount matrix: |
| 50% discount for LCRC Program Members |
| 30% discount for LCRC Contributing Members |
| 10% discount for LCRC Associate Members |
| 0% discount for non-members |
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MSC Propagation and Expansion Cost: $960
Estimated cost to grow 107 MSC’s at p2
Charges based on the purchase price for one vial of 106 frozen p1 MSC’s from the GMP facility, plus the cost of propagating the cells in vitro (cost of materials only: e.g. medium, serum, plasticware etc.)
Colony Forming Unit (CFU) Assay - Cost: $13
Charges based on cost of materials and reagents.
Differentiation Assays - Cost: $76
Charges based on cost of materials and reagents.
Stem Cell Storage - Cost not yet determined.
Please contact us if you are interested in this service.
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Forms
All the forms are in Adobe PDF format. You will need Adobe Reader to open these files. If you do not have Adobe Reader click on the Get Adobe Reader link below to install it in your computer.
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For more information on the Core, Contact:
Director: Radhika Pochampally, Ph.D.
Assistant Professor of Pharmacology, Tulane
Co-Director: Brian G. Rowan, Ph.D.
Associate Professor of Structural and Cellular Biology, Tulane
Manager/Lead Technician: Jessica DeVay, B.S.
email: asccf@lcrc.info
Phone: 504-988-3029
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